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Fittings for HPLC

16 Solvents and solutions that should be avoided when using PEEK fittings or tubing.

The solvents and chemicals that should be avoided when using PEEK tubing and fittings include: Nitric acid Sulfuric acid Halogenated acids, including hydrofluoric, hydrobromic, and hydroiodic acids Pure…

17 What are the critical dimensions of ferrules for low pressure tubing?

The bore size of the ferrules (catalog# 45216-F-ETFE or 45216-F-PTFE) is ID 1.70 ± 0.05 mm, designed for use with 1/16th" OD tubing. © Copyright MicroSolv Technology Corporation, all rights reserved.…

18 What are the temperature and pressure specifications for stainless steel tubing?

It is very hard to claim any particular pressure or temperature rating for Stainless Steel tubing used in HPLC applications without knowing the specifics of use as there are many types of SS tubing used…

19 What does stainless in stainless steel mean?

According to Wikepedia, stainless steel does not corrode, rust or stain like other carbon steel does due to the chromium added to the steel. Wikedpedia article about stainless steel © Copyright MicroSolv…

20 What does the term 10-32 mean? What does it mean to a chromatographer working with HPLC?

This is a way of describing the threading on the fitting so that it will properly be matched to the receiving port. This is important for a good seal and long life of the fittings. Improper use will cause…

21 What is a fitting? I hear the term used all the time but I would like to know what the specific definition is.

The dictionary defines a fitting as “a small often standardized part used to join other parts together properly.” This is very similar to what plumbers refer as fittings. For the use of chromatographers,…

22 What is the appropriate stop depth/seating depth for your HPLC column end fittings?

The stop depth or seating depth is the distance between the end of a ferrule and the end of a section of tubing in the inlet port of the column. If the stop depth is too short, there will be a gap between…

23 What is the best way to attach the inlet of the knitted reactor coil tubing to the outlet of a tee with a PEEK finger tight fitting that will hold high back pressure?

Confirm that there is not an impediment to reasonable flow through the tubing which could increase the back pressure. We recommend using our items 47320-30 and 47320-34 which are High pressure Male nuts…

24 What is the difference between a Luer Lock and a Luer Slip Connector?

When using a device such as a syringe filter and a syringe which has a Luer slip connector on it, the device will simply slide onto the tip of the syringe. If it is applied properly (fully placing the…

25 What is the difference between a union and an adapter in HPLC?

The biggest difference is the price where a union is much less expensive than an adapter. For performance considerations, a union will make a connection between tubing and fittings that have the same…

26 What is the difference between ferrules made of stainless steel vs. stainless steel/PEEK hybrids?

Stainless steel ferrules are used when: • Absolute maximum operating pressure is required, in excess of 15,000 PSI. • The connection is ‘permanent’; that is, the connection will not be made and…

27 What is the inside diameter ID of standard tubing used in that is most suitable for HPLC mobile phase? FAQ

There really is no standard size and this is truly a preference to meet your method objectives. For HPLC, on the high pressure side, 1/16th inch OD is most commonly used and for the low pressure side…

28 What is the maximum pressure that the MC Fittings Universal Column Connectors are specified for?

The MC Fittings, universal HPLC column connectors are rated up to 6000 psi. © Copyright MicroSolv Technology Corporation, all rights reserved.

29 What is the maximum temperature that the MicroSolv Endure fittings can be used in?

150°C for Endure Carbon PEEK fittings and 100°C for most other PEEK fittings. Click here to view Ordering Information for Male Nuts for HPLC © Copyright MicroSolv Technology Corporation, all rights…

30 What is the pH range of 316 stainless steel laboratory tubing and fittings?

The useful pH range of 316 stainless steel tubing and fittings is 1 - 14. More information about stainless steel tubing © Copyright MicroSolv Technology Corporation, all rights reserved.

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