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Fittings for HPLC

1 Can I use a PEEK finger tight fitting on stainless steel tubing? Can I use a SS fitting on PEEK tubing in HPLC?

Yes, you can very effectively use PEEK fittings on stainless steel tubing as long as you take into account the making pressure ratings of the fitting you are using and the expected pressures in your system.…

2 Contaminants leaching into an analysis, suggestions for troubleshooting.

Have you ever run across a situation where Teflon-like compounds where contaminating an analysis? PFA (PerFlourAlkoxy film, a type of Teflon) is leaching out of something from pipette to HPLC and I have…

3 Do all PEEK HPLC fittings fit all receiving ports?

No, you must consider if the receiving port is metric or English, the thread diameter, the pilot length of the receiving port, the thread count and the pressure expected. If you are not sure if your fitting…

4 Do I need a connector when using the Cartridge Columns that use WVS Hardware?

The WVS (Whatman Void Sealing) cartridge columns use a unique sealing system to ensure compression of the packed bed even if a void develops. All of our WVS cartridge column products require the use of…

5 Do the High Pressure Tee Fittings require ferrules or flat-bottom (flangeless) fittings?

The High Pressure Tee fittings require ferrules. Only the low pressure Tee junctions require the flat bottom fittings. Unions for HPLC Ordering Information © Copyright MicroSolv Technology Corporation,…

6 Fittings defined for HPLC

The dictionary defines a fitting as “a small often standardized part used to join other parts together properly.” This is very similar to what plumbers refer as fittings. For the use of chromatographers,…

7 Fittings definition: Screw Thread Call-Out

What is a Thread Call-out for "English" fittings? This is a common system for describing the threading on a fitting that uses the English method. The Thread Call-Out normally consists of two numbers where…

8 How can my finger tight fitting be producing band broadening in HPLC?

Most chromatographers may not realize it but if fittings are not properly set and a perfect connection is accomplished, a dead volume can occur that does not get swept. This extra volume can cause band…

9 How do I choose the correct HPLC column coupler for my instrument?

We offer a variety of column couplers. To find the correct size, simply match your tubing internal dimension (ID) to the coupler. The different Coupler colors correspond with those of the HPLC tubing…

10 How does the Clean-Cut90™ tubing cutter work for cutting PEEK tubing?

Clean-Cut90™ Tubing Cutter for PEEK Tubing The blade of the Clean-Cut90 tubing cutter has 2 sides to consider. One side (marked on the tool with an arrow) gives a perpendicular cut. The other side of…

11 How one fitting for chromatography differs from others in HPLC.

Of course, the quality of the manufacturing is important but besides that the most important features of all fittings are: Geometry of the receiving port you are putting the fitting into. Threads on the…

12 I am using TFA in HPLC and someone told me that I might have a problem with my Delrin type fittings. Is this true?

That person is correct. Delrin is not compatible with Trifluoroacetic Acid. If it comes in contact with your fittings, it could become compromised and your connection might slip and become less than perfect.…

13 Is PEEK tubing or PEEK fittings autoclavable?

Yes, many scientists have used PEEK successfully when autoclaving PEEK tubing and or PEEK fittings using gamma irradiation, heat or ethylene oxide. PEEK tubing information © Copyright MicroSolv Technology…

14 Is stainless steel tubing or fittings autoclavable?

Yes, many scientists have used 316 stainless steel successfully when autoclaving tubing and or fittings for HPLC using gamm irradiation, heat or ethylene oxide methods. Stainless steel tubing information…

15 Should I use a 1-piece fitting or should I use a 2-piece fitting for HPLC?

This is a matter of choice... The benefits of a 1-piece is that it is convenient and easy to use. The benefits of a 2-piece fitting is that the ferrule is the only piece that needs to be replaced when…

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