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What are the differences between Cogent Amide columns and all the other Amide brands on the market?

Reference Number: AA-03223 Created: 07/07/2018 04:52 PM Last Updated: 09/17/2020 03:05 PM

The Cogent Amide HPLC column is very polar and effective for compounds that have amines as well as sugars such as galactose, sucrose, glucose and basic pharmaceuticals such as Prozac, Tizanidine and others.

The Cogent Amide™ column is made with TYPE-C™ silica so it can be used in Reversed Phase, Normal Phase or Aqueous Normal Phase HPLC and does not suffer from the hydrolysis of the amide group that is well documented in other brands. This is due to the direct silicon carbon bonds used in the synthesis that only exist with Cogent TYPE-C™ columns.

Another major difference between the Cogent Amide column and all other amide columns is that Schiff bases do not deactivate the Cogent columns and the Cogent Amide stationary phase does not create Schiff bases.

Simple carbohydrates like sugars are often separated by using an amino column. The biggest difference between the "amino" columns v. the "amide" columns is that amines are basic and ionizable and amides are not. Also, amide functional groups do not create semi-permanent associations with aldehydes the way amines do. Once the association is created, the column has been modified and is almost impossible to reverse.

Click HERE for Cogent Amide HPLC Column Ordering Information.

    


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