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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

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Normal phase, reversed phase, and ANP definitions

Reference Number: AA-02818 Created: 06/24/2016 02:45 PM Last Updated: 07/23/2016 09:34 PM

Normal Phase Chromatography
Using Normal Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer. 


Reverse Phase Chromatography
Using Reverse Phase Solvents:
• Most retained compounds are least polar.
• Least retained compounds are most polar.
• More Polar Solvents (water) Make compounds retain longer. 


Aqueous Normal Phase Chromatography (ANP)
Using Reverse Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer. 


Cogent TYPE-C Silica™
HPLC silica with a surface populated mostly with Silicon-Hydride functional groups instead of silanols.
• Brand name of MicroSolv Technology Corporation.  

  
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