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Using different Cogent™ HPLC stationary phases for different selectivity.

Reference Number: AA-02555 Created: 02/15/2015 01:46 PM Last Updated: 09/12/2020 02:32 PM

Each of the Cogent™ stationary phase materials has a different bonded ligand and therefore potential for different selectivity of given analytes. Consider the example, featuring the Cogent Bidentate C18™ and Cogent UDC-Cholesterol™ columns in another Knowledge Base article: 

Note the two columns give substantially different retention for the same analytes under the same conditions. As a more hydrophobic material, the Cogent Bidentate C18™ leads to greater retention. However, with the Cogent UDC-Cholesterol™ column, selectivity for anthracene / tert-butylbenzene is enhanced (α = 2.30 vs. 1.58). Hence the Cogent columns can be used as method development tools to obtain different selectivity or retention. This behavior is not limited to reversed phase methods but applies also to Aqueous Normal Phase (ANP) and classical normal phase.

Cogent Bidentate C18 HPLC Column Ordering Information
Cogent UDC-Cholesterol HPLC Column Ordering Information


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