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Fused insert glass vials (cat# 9532S-WCV) break during sample injection

Reference Number: AA-02446 Created: 09/18/2014 01:30 PM Last Updated: 09/15/2020 01:14 PM

Question: When injecting samples using fused insert glass vials, sometimes the needle hits the insert wall, causing it to break. Are the inserts not aligned vertically?

Answer: This is more likely due to the needle not being aligned vertically rather than the insert wall itself. This can occur for a number of reasons, which may include:

- Too large a needle gauge. If the gauge number is too high, the needle may be too thin and more easily deflected/bent
- Tip style is incorrect for the septa (i.e. beveled vs. flat tip).
- Septa hardness and/or inconsistency bends or deflects needle. Use partial-slit or soft guard septa in this case.
- Use wide-opening vials with fused insert. Here the needle is less likely to hit the side because there is more room.

    Other possibilities:

- Reset the datum indexer on the autosampler. This is the “Zero Home” or similar function in the software.
- The needle may be over-driven into the bottom of the insert, causing it to break. Reset the travel depth and/or use our inserts with a spring.

     Inserts Product Page 

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