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Peaks are broad and non-symmetrical in LC-MS method using Reversed Phase HPLC

Reference Number: AA-02330 Created: 05/19/2014 01:40 PM Last Updated: 07/23/2016 03:47 PM

There are various possible solutions using Cogentâ„¢ HPLC columns:

1. Diluent: Is your diluent too strong for the mobile phase? You should not have a 90% acetonitrile diluent with a 10% acetonitrile reversed phase method. You may observe peak distortion for this reason.

2. Mobile phase/diluent pH: Do you have any basic analytes? If so, perhaps they are responsible for the poor peak shape. You can operate at an acidic mobile phase pH where any residual silanols are neutral, avoiding electrostatic interactions with basic compounds.

3. Sample Concentration/Injection Volume: Every HPLC column has a maximum loading capacity. If too much analyte is introduced to the column, you may see peak distortion due to overload. Try reducing either concentration or injection volume. 

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