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Acetone as the Organic Mobile Phase Component

Reference Number: AA-01078 Created: 12/19/2012 01:06 PM Last Updated: 06/07/2017 12:35 PM

“Green” substitute for separation of amino acids 

 

Caution: Acetone and Amines can react  When working with Acetone as an HPLC solvent it is important to note that primary amines could react with Acetone to form imines. However, to achieve this reaction the mobile phase would most likely need to be free of water and or acid as a catalyst. It is reported by the University of Liverpool that "Imines are formed when any primary amine reacts with an aldehyde or ketone under appropriate conditions. Imine formation requires an acid catalyst, otherwise the reaction is very slow. the acid is needed for the elimination of water."

Working with the above and other methods in ANP, we have not seen the formation of imines occur.
   

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