Organic Alcohols Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9184)
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November 28, 2012
Bill Ciccone

AppNotes Chemicals/Energy / AppNotes Cogent Bidentate C18 / AppNotes Library / AppNotes Listed by Discipline / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Separation of 1-Phenyl-2-Propanol and 2-Phenyl-1,2-Propanediol

In this Method, Separation is obtained between two structurally similar Organic Alcohols. The Method illustrates the type of Retention and Selectivity that can be obtained for these kinds of compounds. Furthermore, the Mobile Phase Solvents are simple to prepare and LCMS compatible.

The overlay of the 5 Figures demonstrates how the Method is Precise and Reliable when the Column is changed with lot-to-lot, which is a critical feature. RSD values of 0.5% and 0.3% were obtained for the two Peaks between the 5 lots.

Peaks:
1. 1-Phenyl-2-Propanol
2. 2-Phenyl-1,2-Propanediol

Method Conditions:
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0 .1% Formic Acid (v/v)
—B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	10
2	10
6	50
7	10

Injection vol.: 5 µL
Flow rate: 1.0ml / minute
Detection: UV @ 220nm
Post time: 3 minutes
Sample Preparation: 0.1µL / mL 1-Phenyl-2-Propanol and 0.1mg / mL 2-Phenyl-1,2-Propanediol in Diluent of 50:50 Solvent A / Solvent B.
t0: 0.9 minutes

Note: These two compounds are used as starting materials in organic syntheses. As such, the Method has relevance in impurities testing of finished products to ensure no starting materials are present.

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