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How do I choose an appropriate guard column for my method using Cogent TYPE-C HPLC columns?

Reference Number: AA-00928 Created: 07/25/2012 02:57 PM Last Updated: 07/22/2016 05:01 PM


Two sizes of guard columns are available: 2.0 and 4.0 mm internal diameter (ID). Both sizes are 20mm in length and are used with a guard column holder. The 2.0mm ID guard columns are for use with the 2.1mm ID analytical columns and the 4.0mm ID guard columns should be used with the 3.0mm and 4.6mm ID analytical columns.

Typically one would use the same stationary phase type in both the guard and analytical columns. However, in some instances it is advisable to use a reversed phase guard column (such as the Bidentate C18™) with a Diamond Hydride™ analytical column. It depends on which types of analytes from the matrix one expects to be most problematic in terms of carryover and adsorption on the analytical column.

     Cogent Guard Column Information 


   
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