MicroSolv Technologies Knowledge Base

Microsolv

Browse Ask a Question
 
Tools
Categories

Using Aqueous Normal Phase (ANP) and Aqueous Reverse Phase (ARP) on One TYPE-C™ Based HPLC Column

Reference Number: AA-00866 Created: 05/23/2012 04:42 PM Last Updated: 07/12/2013 04:06 PM

Swap Peak Order… Can Your HPLC Column Do This?  

Discussion

Chromatograms A & B
Peak 1 (Metformin) eluted with longer retention time as the concentration of Acetonitrile was increased. During the same run, Peak 2 (Glyburide) elutes with shorter retention time as concentrations of Acetonitrile are increased.

Attachments
No 2 Using Aqueous Normal Phase Hi Rez.pdf 4 Mb Download File
Info Ask a Question 
Your Email: Subject: Question: