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3,3'-Diaminobenzidine (DAB) by LC-MS

Reference Number: AA-00818 Created: 05/18/2012 06:44 AM Last Updated: 07/12/2013 12:15 PM

LC-MS method with excellent retention and peak shape  

Discussion

DAB (3,3'-Diaminobenzidine) is a very challenging compound for analysis by HPLC. It is highly polar and hence difficult to retain when RP-HPLC columns are used. Moreover, when there are a significant number of silanol groups present on the surface of the column packing material, the peak for DAB becomes very broad (5 – 10 min peak width). As can be seen from the accompanying chromatograms, a Cogent Diamond Hydride™ column was an excellent choice for the analysis of DAB. The peak shape is symmetrical with high efficiency. The repeatability of the analysis is also remarkable as can be seen in Figure B.

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No 151 Diaminobenzidine by ANP LCMS DH.pdf 0.3 Mb Download File
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