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Trans-3-hydroxycinnamic acid in Human Urine

Reference Number: AA-00761 Created: 05/15/2012 07:15 AM Last Updated: 07/12/2013 10:44 AM

Separation in LCMS using the Diamond-Hydride  

Discussion

Polar metabolites are reproducibly retained using an MS friendly, ANP (inverse gradient) mobile phase. The sample preparation required is very easy and cost effective ($3 per sample to $0.35 per sample of urine) compared to ordinary methods. The sample is diluted with the ANP solvents and ready to inject in 5-10 minutes. With this method, investigating metabolite excretion of laboratory animals or determining endogenous urinary biomarkers or working on phenolic composition of plant extracts is simple and easy.

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No 56 Antioxidants in Human Urine.pdf 1.5 Mb Download File
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