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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

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Trans-3-hydroxycinnamic acid in Human Urine

Reference Number: AA-00761 Created: 05/15/2012 07:15 AM Last Updated: 07/12/2013 10:44 AM

Separation in LCMS using the Diamond-Hydride  


Polar metabolites are reproducibly retained using an MS friendly, ANP (inverse gradient) mobile phase. The sample preparation required is very easy and cost effective ($3 per sample to $0.35 per sample of urine) compared to ordinary methods. The sample is diluted with the ANP solvents and ready to inject in 5-10 minutes. With this method, investigating metabolite excretion of laboratory animals or determining endogenous urinary biomarkers or working on phenolic composition of plant extracts is simple and easy.

No 56 Antioxidants in Human Urine.pdf 1.5 Mb Download File
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