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Diphenhydramine HCl HPLC method and application note

Reference Number: AA-00699 Created: 05/10/2012 05:12 PM Last Updated: 07/12/2013 02:27 PM

Online SPE with a Simple RP Mobile Phase  


When a mobile phase containing 80%B (with 10 mM pyridine in solvent A and B) was used diphenhydramine hydrochloride (DPH) was fully retained on the column. After the mobile phase was changed to the one described above, the DPH peak eluted at 3.5 minutes. This wide range of retention time can give analysts many possibilities of developing methods for quantitative analysis of formulations, drugs and also on column SPE methods for isolation of DPH from complex matrices.

The method above is very reproducible and sensitive with a lower limit of quantitation (LLOQ) of 5 ng/mL for DPH, with good linearity in the range 1- 500 ng/mL (r2 > 0.9990).

No 79 Diphenhydramine by LCMS online SPE.pdf 64.4 Kb Download File
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