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Leucine & iso Leucine branch chain amino acids

Reference Number: AA-00690 Created: 05/10/2012 07:11 AM Last Updated: 10/31/2013 09:45 AM

Leucine, Isoleucine

Discussion

The chromatogram presented was done using a Cogent Diamond column and an ANP gradient. The addition of weak acetic acid to the mobile phase allows the small difference in the pKas of the two compounds to be used to achieve the resolution for this pair of analytes which have identical m/z values. A few microliters of serum suffices to estimate the leucine and isoleucine present. The two branched-chain amino acids are separated and can be easily quantified. The method is especially applicable to the frequent monitoring of serum in treating patients with maple-syrup disease, and may also be used for rapid diagnosis of the disease in suspected infants.

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No 84 Leucine & Isoleucine BCAA on DH.pdf 0.8 Mb Download File
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