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HILIC v ANP at high flow rate on standard HPLC equipment

Reference Number: AA-00664 Created: 05/07/2012 07:13 AM Last Updated: 07/12/2013 11:08 AM

Very high flow rate, highe efficiency & selectivity on standard HPLC equipment

Discussion


Figure A shows a plot of “Flow Rate vs. HETP” for two carbohydrates analyzed using a 2.1 x 150 mm column. From the plot it is evident that while the flow rate is increased by a factor of 2.5 the efficiency is only slightly lower (~15%). When a 1 mm column was used over the same flow rates (linear flow rates more than four times higher) the decrease in efficiency was only 20%.

Figure B shows a plot of “Efficiency vs. Flow Rate” for uracil using a 4.6 x 150mm column. From the plot it can be seen that efficiency increases as the flow rate increases from 0.3 to 1.0 mL/min. In contrast when a market leading HILIC column was used under the same conditions, the efficiency dropped by more than 30% over this same range of flow rates. It should be noted that linear flow rates are more than 6 times greater in the 2.1 mm column than in the 4.6 mm.

 
 
 
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No 108 High Flow Rate & Efficiency in ANP.pdf 0.2 Mb Download File
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