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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

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Urea analysis

Reference Number: AA-00663 Created: 05/07/2012 07:08 AM Last Updated: 07/12/2013 04:06 PM

Fast, reproducible method


A Cogent Bidentate C18™ column was successfully used to retain Urea when a 100% DI water mobile phase was used. Since this hydrophobic column was made on TYPE-C Silica™ DI water can be used as the complete mobile phase without fear of phase “dewetting” and subsequent loss of retention time. The presented method has the advantage of being very simple, without time consuming derivatization or reaction steps when compared to other methods using ordinary C18 columns. Urea can be determined in complex mixtures using this very fast, reproducible (seeinsert on the chromatogram) method which could also be useful in the analysis of biological samples for diagnostic purposes.

No 111 Analysis of Urea on BC18 in RP.pdf 0.2 Mb Download File
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