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Folic acid content in fortified cereals

Reference Number: AA-00651 Created: 05/03/2012 06:58 AM Last Updated: 07/12/2013 11:13 AM

Online Cleanup of Sample or Matrix Components


This application note demonstrates an effective means of separating and resolving folic acid from undesired matrix components in cereal extracts without the use of SPE or other sample cleanup techniques. SPE is normally performed to remove compounds from the matrix which would co-elute with folic acid before injection but by performing the separation shown above, this step can be virtually omitted. Many of the matrix components in this cereal are less polar than folic acid and therefore elute earlier in the HPLC run. In this manner, the separation can act as an online sample cleanup and eliminate chances of losing any folic acid during this step. This helps to provide better reproducibility.

The precision of this method is clearly demonstrated by the low %RSD (0.1% and below) of the folic acid retention times. The calibration curve showed good linearity (R
2 = 0.9997).

Results obtained by this method agree with the amount of the folic acid reported by the manufacturer. Methotrexate was used as an internal standard for the developed method.



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