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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

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Prednisone quantitation method

Reference Number: AA-00647 Created: 05/02/2012 04:41 PM Last Updated: 07/12/2013 04:12 PM

Simple and easy with LCMS detection


The LC/MS method in this note does not need any type of derivatization and has therefore offers a great advantage over other HPLC methods. Two types are gradients are presented which will allow you to choose one depending on other components in the sample and your method objectives. Retention can be achieved on a very short column (50 mm) as shown.  Linear gradient conditions were used for analysis of this important corticosteroid. All TYPE-C Cogent columns equilibrate very rapidly to initial conditions when gradient analysis is used.

No 123 Quantitation of Prednisone BC18 RP.pdf 0.1 Mb Download File
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