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I am analyzing metformin in plasma samples using acetonitrile + DI water with 0.5% formic acid. How I can obtain reproducible results and peak shape?

Reference Number: AA-00578 Created: 04/22/2012 05:32 PM Last Updated: 07/23/2016 03:17 PM

You can try substitute formic acid in DI water by adding ammonium acetate (10 mM) and acetic acid (1%) to the aqueous phase. This should improved the reproducibility of peak areas and retention times. In addition extensive reconditioning of the analytical column with pure acetonitrile in between separations is essential for good reproducibility when working with plasma samples. 

   
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