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What are the typical flow rates recommended by HPLC column companies to start with, for various column ID’s?

Reference Number: AA-00575 Created: 04/22/2012 05:27 PM Last Updated: 07/23/2016 05:03 PM

Although it will vary from column maker to column maker, and is very dependent on the column length back pressure the following is good rule of thumb for common columns.

Column Dimensions
Recommended Flow Rate
1.0
30-60µL/min
2.1
0.1-0.6mL/min
3.0
0.3-1.5mL/min
4.6
0.8-3.0ml/min
7.8
4.0-10mL/min



   
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