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How can my finger tight fitting be producing band broadening in HPLC?

Reference Number: AA-00447 Created: 04/21/2012 02:15 PM Last Updated: 09/12/2020 03:41 PM

Most chromatographers may not realize it but if fittings are not properly set and a perfect connection is accomplished, a dead volume can occur that does not get swept. 

This extra volume can cause band broadening or ghost peaks or may decrease the sensitivity of your run by increasing baseline noise.

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