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How to change columns from reversed phase to normal phase mode and vice versa

Reference Number: AA-00403 Created: 04/11/2012 08:31 PM Last Updated: 07/23/2016 02:46 PM

Just as a reminder, all Cogent TYPE-C™ columns are filled with water containing solvents during shipping. To start work in normal phase mode, a simple switching procedure is recommended.

A simple 30 minute procedure allows switching from one mode to another.

Procedure:

A – moving from Reverse Phase to Normal Phase HPLC; pump 100% methanol for 15 minutes at 1 mL/min. flow rate, followed by 15 minutes 100% methylene chloride. The column is ready to be equilibrated with mobile phase for NP-HPLC.

B – moving from Normal Phase to Reverse Phase HPLC; pump 100% methylene chloride for 15 minutes at 1 mL/min. flow rate, followed by 15 minutes 100% methanol. The column is ready to be equilibrated with mobile phase for RP-HPLC. 

   
    
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