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How to Calibrate UV-vis and HPLC Detectors with NIST Traceable Solutions Below 230nm

Reference Number: AA-00398 Created: 04/10/2012 09:31 PM Last Updated: 04/10/2012 09:40 PM

Holmium Oxide is an internationally recognized wavelength calibration primary standard, supported by NIST and other standards organizations. It provides 14 official absorbance bands ranging from 241 nm to 641 nm. Caffeine is considered a secondary standard, with two absorbance bands at 205 nm and 273 nm. Caffeine is also widely used as a wavelength and absorbance standard.

The MicroSolv HSQ Kit™ has been designed so that the combination of Caffeine with Holmium Oxide, will provide NIST-Traceable wavelength calibration down to 205 nm. First using Holmium Oxide, the detector is calibrated as described in the instructions, at 241 nm and above. Next, the wavelengths of Caffeine are determined at 205 nm and 273 nm. NIST traceability is achieved by the 273 nm band, which overlaps the official NIST range. Assuming that the detector meets at this wavelength, the 205 nm band becomes NIST traceable, and can be used with confidence to ascertain the wavelength accuracy of the detector at this lower wavelength. Using these two standards in combination with each other, provides a means to qualify your detector from 205 nm to 641 nm with full confidence.

 

 

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