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HSQ High Speed for HPLC

1 Are column oven temperatures qualified by the HSQ Kit?

No, the use of column ovens are not required for PQ Testing of your HPLC instruments. © Copyright MicroSolv Technology Corporation, all rights reserved.

2 Can I choose mobile phase percentages other than those used in the HSQ Spreadsheet?

The HSQ Kit gradient test calls for mobile phase compositions of 100/0% and 90/10% (for each of up to four solvent pairs). The user then inputs the absorbance values at each portion of the gradient into…

3 Can I get MSDS (Material Safety Data Sheets) for the HSQ Kit solutions?

Yes, they are available to all customers that purchase the HSQ Kit™ or you can download them from our website at http://www.mtc-usa.com/msds.asp © Copyright MicroSolv Technology Corporation, all rights…

4 Can I measure the extra column volume of my system and compare it to extra column volume of another instrument?

Yes, that is one of the added features of the MicroSolv HSQ Kit™. Using the Rs Test Mixture, and the Excel template, using sophisticated algorithms, a very good estimation of your instruments extra…

5 Can I use qualification solutions with different lot numbers?

If you require or would like to use a mix of different lot numbers for solutions from the MicroSolv HSQ™, UV-vis™ or PQ™ Kit solutions, this is an acceptable protocol under some conditions: You…

6 Can I use the holmium oxide solution (PQA-HOX-01) in a UV instrument that calls for Starna or NIST cuvettes?

If your instrument manual calls for the SRM 2034 Holmium Oxide (HOX) Solution, you can use our HOX solution instead. The SRM 2034 item has been discontinued by NIST but our HOX solution is fully NIST…

7 Can I use the HSQ Kit for IQ and OQ as well as PQ?

From USP <1058>: Installation Qualification (IQ) - The documented collection of activities necessary to establish that an instrument is delivered and designed as specified, and is properly installed…

8 Can I use the HSQ Kit with the longer column from the original PQ Kit?

If you're required to use the older 150mm PQ Kit column and cannot use the 75mm HSQ Kit Column, you can adjust the parameters in the Excel Spreadsheet shown here: Change Flow Rate = 1 mL/minute Change…

9 Can I use the MicroSolv HSQ kits to qualify a UHPLC instrument?

Fundamentally, UHPLC is no different from conventional HPLC. All of the same principles apply, only UHPLC uses higher pressure pumps, lower extra-column system volume, and so on. You can even use the…

10 Can I use the standard, 4um HPLC column supplied in the HSQ Kit for UHPLC instrument qualification?

Yes, the MicroSolv HSQ Kit comes with a 4um particle size HPLC column that can be used to qualify a UHPLC instrument; you do not need a 2um packing UHPLC column. Many qualification parameters are detector…

11 Can I use the UV Calibration Kit to qualify detection at 200nm?

The caffeine standard provides two data points at 205 and 273nm. The holmium oxide solution covers 14 absorbance bands in the range of 241 - 641nm. Hence, you can obtain a calibration curve from these…

12 Can the HSQ Kit be used to measure the LOQ and LOD differences between instruments in my lab?

Yes, this is another really great “extra feature” of the HSQ Kit™. By determining the, signal to noise ration, the extra column volumes and the dwell volume of each instrument using the HSQ Kit…

13 Can the L3 linearity solution supplied in the HSQ Kit be used for UV wavelength accuracy?

The short answer is yes. Many other vendors use only caffeine for wavelength accuracy, at 205 and 273 nm. Holmium oxide of course covers 241-641nm, and is directly traceable to NIST standards. However,…

14 Do the different tests in the HSQ Kit use the same method conditions?

Yes, this is why it can be run overnight from an autosampler. Simply set your conditions, program the system for PQ Validation and let it run. Use the following Conditions: Wavelength: 273nm Injection…

15 Do you supply an SOP template with the HSQ Qualification Kit

We no longer supply a formal template or extensive written SOP for Performance Qualification. However, the Excel template that is provided with the HSQ (High Speed Qualification) kit can be a model for…

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