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1 Can Cogent TYPE-C columns be used for high temperature analysis?

Cogent™ HPLC columns are made with state of the art, modular, column hardware. The frits are stainless steel inserted in a high quality PEEK cap device and positioned onto the column during manufacturing.…

2 Can I use methanol instead of acetonitrile in my HPLC method?

Methanol is a very common choice for the HPLC mobile phase organic solvent component in reversed phase HPLC even though acetonitrile is often superior in several aspects. On the other hand, methanol is…

3 Does MicroSolv offer TYPE-C HPLC columns that do not have PEEK components

Most of our Cogent™ HPLC columns are made with state of the art, column hardware that contains stainless steel frits that are held to the column during manufacturing with caps that are made with high…

4 How do nano, capillary, and micro-LC differ in terms of column ID and typical flow rates?

The following table shows the flow rate and column ID ranges for the various terms which is generally accepted by chromatographers but definitions may differ from different sources. Term Colum i.d. range…

5 Maximum temperature for Cogent Diamond Hydride HPLC columns

The maximum operating temperature for the Cogent Diamond Hydride™ HPLC column is 60° C. However, higher temperature columns are available on request with high temperature hardware. Cogent Diamond Hydride…

6 pH range for Cogent Diamond Hydride HPLC columns.

The recommended pH range for the Cogent Diamond Hydride™ HPLC column is 2.5-7.0. Cogent Diamond Hydride Specifications © Copyright MicroSolv Technology Corporation, all rights reserved.

7 What are theoretical plates and HETP?

The chromatographic process is comprised of a continuous series of equilibria in which the solute is interacting either with the mobile phase or the stationary phase. These interactions may be approximated…

8 What is a "base-deactivated" HPLC column?

"Base-deactivated" means that the column is designed for analysis of basic compounds. Conventional columns based on type B silica typically have Si-OH groups on the surface that interact with bases. This…

9 What is included with the Whatman SAX HPLC column kit (cat# 63185-SCK)?

The product consists of: 1 each Partisil™ 10um SAX 250 x 4.6mm id standard analytical HPLC column 1 each Solvecon™ precolumn kit for above: 15g Solvecon™ silica, empty 250 x 4.6mm id column (including…

10 What is the bonded phase of the Cogent F5™ HPLC column and which applications is it used?

The Cogent F5™ HPLC column is a pentafluoro phenyl (PFP) phase based on high purity type B silica. PFP columns are considered legacy technology in that they offer advantages for specific types of analytes…

11 What is the maximum pressure Cogent HPLC columns will tolerate?

Cogent™ HPLC Columns with 4 & 5um particles are rated up to 6,000 psi. However, it is not recommended (by most instrument producers) that analyses are carried out at this high level. No more than…

12 What is the maximum pressure of the PEEK HPLC columns?

The Cogent™ 2.1mm ID column is made of 1/8” tubing.The maximum recommended pressure of the PEEK HPLC column under routine use is 165 bar (2400 psi). However, when packing the stationary phase, a pressure…

13 What problems can occur when using a dedicated Reversed-Phase HPLC instrument for Aqueous Normal Phase (ANNP) methods?

When a system is constantly run in Reversed-Phase (RP) HPLC, many contaminants are retained or coat seals, frits, check valves and many other parts of an HPLC system. When these contaminants build up,…

14 Where do I buy Cogent HPLC Columns?

Depending on your location, Cogent HPLC columns are available worldwide through MicroSolv or through distribution. To determine where you should buy these columns see the list below. United States Direct…

15 Which Guard Column do I use with the Partisil™ SAX Columns?

We suggest you use catalog # 66900-AGC (Anion Guard Cartridge (5 pack)), for use with Hichrom Partisil™ (formerly Whatman GE Partisil) HPLC columns. You will also need a holder which is our catalog…

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